stop buffer (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc stop buffer
Stop Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stop+buffer/CUT%26RUN+Stop+Buffer/pm41456056-230-4-9
Average 93 stars, based on 17 article reviews

stop buffer - by Bioz Stars, 2026-09

93/100 stars

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Article Title: The formation and propagation of human Robertsonian chromosomes
Article Snippet: The enzymatic digestion was terminated by adding 1× stop buffer (prepared from 4× stop buffer (Cell Signaling Technology, 48105), digitonin solution and 200× RNase A (Cell Signaling Technology, 7013)).

Article Title: The formation and propagation of human Robertsonian chromosomes.
Article Snippet: The enzymatic digestion was terminated by adding 1× stop buffer (prepared from 4× stop buffer (Cell Signaling Technology, 48105), digitonin solution and 200× RNase A (Cell Signaling Technology, 7013)).

Incubation:

Article Title: Eomesodermin in conjunction with the BAF complex promotes expansion and invasion of the trophectoderm lineage.
Article Snippet: Beads were washed with digitonin buffer (wash buffer with 0.02% Digitonin) before incubation with pAG-MNase (Cell Signalling Technology, 40366) in digitonin buffer for 1 h at 4 °C, followed by two washes in digitonin buffer. .. Chromatin was released by the addition of STOP buffer (340mM NaCl, 20mM EDTA, 4mM EGTA, 0.02% Digitonin, 100 μg/ml RNAse A, 50 μg/ml Glycogen with Spike-in DNA (CST, 40366)) and incubated at 37 °C for 30min. .. Chromatin was released by the addition of STOP buffer (340mM NaCl, 20mM EDTA, 4mM EGTA, 0.02% Digitonin, 100 μg/ml RNAse A, 50 μg/ml Glycogen with Spike-in DNA (CST, 40366)) and incubated at 37 °C for 30min.

Article Title: Convergent DNA methylation abnormalities at enhancers and bivalent promoters in human growth disorders
Article Snippet: .. After chromatin digestion, the stop buffer (Cat # 48,-105, Cell signaling Technology) and 1 ng E.coli spike-in DNA (Cat # 18–1401, EpiCypher) were added and incubated for 10 min at 37 °C. .. CUT&RUN libraries were prepared with the CUT&RUN library Prep kit (Cat# 14–1001, EpiCypher) following the manufacturer’s instructions.

Article Title: Convergent DNA Methylation Abnormalities at Bivalent Chromatin in Human Growth Disorders
Article Snippet: .. After chromatin digestion, the stop buffer (Cat # 48105, Cell signaling Technology) and 1 ng E.coli spike-in DNA (Cat # 18-1401, EpiCypher) were added and incubated for 10min at 37 °C. .. CUT&RUN libraries were prepared with the CUT&RUN library Prep kit (Cat# 14-1001,, EpiCypher) following the manufacturer’s instructions.

Article Title: Convergent DNA methylation abnormalities at enhancers and bivalent promoters in human growth disorders.
Article Snippet: .. After chromatin digestion, the stop buffer (Cat # 48105, Cell signaling Technology) and 1 ng E.coli spike-in DNA (Cat # 18- 1401, EpiCypher) were added and incubated for 10min at 37 °C. .. CUT&RUN libraries were prepared with the CUT&RUN library Prep kit (Cat# 14-1001, EpiCypher) following the manufacturer’s instructions.

Purification:

Article Title: Nuclear Receptor Transcription Factors promote axon regeneration in the Adult Corticospinal Tract
Article Snippet: Bead–nuclei complexes were washed and exposed to pAG-MNase [CST, #15338] in digitonin buffer [CST, #16359] containing spermidine and protease inhibitors for 1 h, then activated with CaCl₂ for 30 min at 4 °C. .. Digestion was stopped with kit Stop Buffer [CST, #48105] plus RNase A [CST, #7013], and cross-links were reversed with 10 % SDS and Proteinase K [CST, #10012] at 65 °C for 2 h. DNA was purified using kit spin columns [CST, #14209]. .. An input control followed the same workflow without antibody; nuclei were lysed in DNA Extraction Buffer [CST, #42015], sonicated to 100–600 bp on a Covaris M220 (peak 75, duty 10, 200 cycles, 75 s) and processed in parallel.

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